Applications of quantum dots in medical diagnostics - Sideversjonshistorikk

Oyangen på 26. mar. 2009 kl. 10:35

2009-03-26T10:35:55Z

← Eldre sideversjon		Sideversjonen fra 26. mar. 2009 kl. 10:35
Linje 56:		Linje 56:
	Photodynamic therapy uses singlet oxygen, excited from a photosensitizer activated by light, to degrade cellular components, for example in cancer cells. QDs can be used in this treatment by being the photosensitizer itself, or they can be the energy donor, activating another photosensitizer.		Photodynamic therapy uses singlet oxygen, excited from a photosensitizer activated by light, to degrade cellular components, for example in cancer cells. QDs can be used in this treatment by being the photosensitizer itself, or they can be the energy donor, activating another photosensitizer.
	The nature of QDs makes us able to monitor different components in a cell culture over extended periods of time. This is used for example in drug discovery, where possible drugs are monitored simultaneously.		The nature of QDs makes us able to monitor different components in a cell culture over extended periods of time. This is used for example in drug discovery, where possible drugs are monitored simultaneously.

Oyangen på 26. mar. 2009 kl. 10:35

2009-03-26T10:35:35Z

← Eldre sideversjon		Sideversjonen fra 26. mar. 2009 kl. 10:35
Linje 56:		Linje 56:
	Photodynamic therapy uses singlet oxygen, excited from a photosensitizer activated by light, to degrade cellular components, for example in cancer cells. QDs can be used in this treatment by being the photosensitizer itself, or they can be the energy donor, activating another photosensitizer.		Photodynamic therapy uses singlet oxygen, excited from a photosensitizer activated by light, to degrade cellular components, for example in cancer cells. QDs can be used in this treatment by being the photosensitizer itself, or they can be the energy donor, activating another photosensitizer.
	The nature of QDs makes us able to monitor different components in a cell culture over extended periods of time. This is used for example in drug discovery, where possible drugs are monitored simultaneously.		The nature of QDs makes us able to monitor different components in a cell culture over extended periods of time. This is used for example in drug discovery, where possible drugs are monitored simultaneously.



	==Technical limitations in use of QDs<ref>From diagnostics to therapy: Prospects of quantum dots; Hassan M.E Azzazy, Mai M.H Mansour, Steven C. Kazmierczak (2007)</ref>==		==Technical limitations in use of QDs<ref>From diagnostics to therapy: Prospects of quantum dots; Hassan M.E Azzazy, Mai M.H Mansour, Steven C. Kazmierczak (2007)</ref>==

Oyangen: /* Nucleic acid detectionDetection of single DNA molecules by multicolor quantum-dot end-labeling (2005) */

2009-03-26T10:34:45Z

Nucleic acid detectionDetection of single DNA molecules by multicolor quantum-dot end-labeling (2005)

← Eldre sideversjon		Sideversjonen fra 26. mar. 2009 kl. 10:34
Linje 26:		Linje 26:

	===Nucleic acid detection<ref>Detection of single DNA molecules by multicolor quantum-dot end-labeling (2005) </ref>===		===Nucleic acid detection<ref>Detection of single DNA molecules by multicolor quantum-dot end-labeling (2005) </ref>===

	QDs can also be used in fluorescence in situ hybridization as labels for detection of DNA. This use of QDs overcomes the two problems of classic organic fluorescent labeling: Cleavage DNA molecules due to photo bleaching and subsequent formation of free radicals. They also allow two color determination of the orientation of a single molecule.		QDs can also be used in fluorescence in situ hybridization as labels for detection of DNA. This use of QDs overcomes the two problems of classic organic fluorescent labeling: Cleavage DNA molecules due to photo bleaching and subsequent formation of free radicals. They also allow two color determination of the orientation of a single molecule.
	~~[[Bilde:DNA2.JPG\|right\|300px\|]]~~

	===Detection of genetic polymorphism===		===Detection of genetic polymorphism===

Oyangen: /* Nucleic acid detectionDetection of single DNA molecules by multicolor quantum-dot end-labeling (2005) */

2009-03-26T10:33:27Z

Nucleic acid detectionDetection of single DNA molecules by multicolor quantum-dot end-labeling (2005)

← Eldre sideversjon		Sideversjonen fra 26. mar. 2009 kl. 10:33
Linje 26:		Linje 26:

	===Nucleic acid detection<ref>Detection of single DNA molecules by multicolor quantum-dot end-labeling (2005) </ref>===		===Nucleic acid detection<ref>Detection of single DNA molecules by multicolor quantum-dot end-labeling (2005) </ref>===

			QDs can also be used in fluorescence in situ hybridization as labels for detection of DNA. This use of QDs overcomes the two problems of classic organic fluorescent labeling: Cleavage DNA molecules due to photo bleaching and subsequent formation of free radicals. They also allow two color determination of the orientation of a single molecule.
	[[Bilde:DNA2.JPG\|right\|300px\|]]		[[Bilde:DNA2.JPG\|right\|300px\|]]
	QDs can also be used in fluorescence in situ hybridization as labels for detection of DNA. This use of QDs overcomes the two problems of classic organic fluorescent labeling: Cleavage DNA molecules due to photo bleaching and subsequent formation of free radicals. They also allow two color determination of the orientation of a single molecule.

	===Detection of genetic polymorphism===		===Detection of genetic polymorphism===

Oyangen: /* Preparation and functionality */

2009-03-26T10:32:28Z

Preparation and functionality

← Eldre sideversjon		Sideversjonen fra 26. mar. 2009 kl. 10:32
Linje 7:		Linje 7:

	===Preparation and functionality===		===Preparation and functionality===
	[[Bilde:qdot.jpg\|left\|thumb\|~~350px~~\|Functionalization of quantum dots]]		[[Bilde:qdot.jpg\|left\|thumb\|400px\|Functionalization of quantum dots]]
	When preparing QDs it is important to keep in mind the physical properties it will get as a finished compound. For instance, biocompatibility, which is the ability of a material to perform with an appropriate host response in a specific application. It is also defined as the quality of not having toxic or injurious effects on biological systems. Examples of how to make QDs biocompatible are silanization, (covering the surface with silane like molecules) and surface exchange with bifunctional molecules. Another strategy is to encapsulate the QD in for instance within phospholipids.		When preparing QDs it is important to keep in mind the physical properties it will get as a finished compound. For instance, biocompatibility, which is the ability of a material to perform with an appropriate host response in a specific application. It is also defined as the quality of not having toxic or injurious effects on biological systems. Examples of how to make QDs biocompatible are silanization, (covering the surface with silane like molecules) and surface exchange with bifunctional molecules. Another strategy is to encapsulate the QD in for instance within phospholipids.
	The functionality of QDs is achieved by giving the surface layer (ZnS) different characteristics. These characteristics enable the QDs to adapt to the desired application by conjugating to a recognition moiety, like antibodies, peptides, oligonucleotides or aptamers. The functionality depends on the biomolecule of interest		The functionality of QDs is achieved by giving the surface layer (ZnS) different characteristics. These characteristics enable the QDs to adapt to the desired application by conjugating to a recognition moiety, like antibodies, peptides, oligonucleotides or aptamers. The functionality depends on the biomolecule of interest

Oyangen: /* Preparation and functionality */

2009-03-26T10:32:04Z

Preparation and functionality

← Eldre sideversjon		Sideversjonen fra 26. mar. 2009 kl. 10:32
Linje 7:		Linje 7:

	===Preparation and functionality===		===Preparation and functionality===
	[[Bilde:qdot.jpg\|left\|thumb\|~~420px~~\|Functionalization of quantum dots]]		[[Bilde:qdot.jpg\|left\|thumb\|350px\|Functionalization of quantum dots]]
	When preparing QDs it is important to keep in mind the physical properties it will get as a finished compound. For instance, biocompatibility, which is the ability of a material to perform with an appropriate host response in a specific application. It is also defined as the quality of not having toxic or injurious effects on biological systems. Examples of how to make QDs biocompatible are silanization, (covering the surface with silane like molecules) and surface exchange with bifunctional molecules. Another strategy is to encapsulate the QD in for instance within phospholipids.		When preparing QDs it is important to keep in mind the physical properties it will get as a finished compound. For instance, biocompatibility, which is the ability of a material to perform with an appropriate host response in a specific application. It is also defined as the quality of not having toxic or injurious effects on biological systems. Examples of how to make QDs biocompatible are silanization, (covering the surface with silane like molecules) and surface exchange with bifunctional molecules. Another strategy is to encapsulate the QD in for instance within phospholipids.
	The functionality of QDs is achieved by giving the surface layer (ZnS) different characteristics. These characteristics enable the QDs to adapt to the desired application by conjugating to a recognition moiety, like antibodies, peptides, oligonucleotides or aptamers. The functionality depends on the biomolecule of interest		The functionality of QDs is achieved by giving the surface layer (ZnS) different characteristics. These characteristics enable the QDs to adapt to the desired application by conjugating to a recognition moiety, like antibodies, peptides, oligonucleotides or aptamers. The functionality depends on the biomolecule of interest

Oyangen: /* Preparation and functionality */

2009-03-26T10:31:32Z

Preparation and functionality

← Eldre sideversjon		Sideversjonen fra 26. mar. 2009 kl. 10:31
Linje 7:		Linje 7:

	===Preparation and functionality===		===Preparation and functionality===
	[[Bilde:qdot.jpg\|left\|thumb\|~~400px~~\|Functionalization of quantum dots]]		[[Bilde:qdot.jpg\|left\|thumb\|420px\|Functionalization of quantum dots]]
	When preparing QDs it is important to keep in mind the physical properties it will get as a finished compound. For instance, biocompatibility, which is the ability of a material to perform with an appropriate host response in a specific application. It is also defined as the quality of not having toxic or injurious effects on biological systems. Examples of how to make QDs biocompatible are silanization, (covering the surface with silane like molecules) and surface exchange with bifunctional molecules. Another strategy is to encapsulate the QD in for instance within phospholipids.		When preparing QDs it is important to keep in mind the physical properties it will get as a finished compound. For instance, biocompatibility, which is the ability of a material to perform with an appropriate host response in a specific application. It is also defined as the quality of not having toxic or injurious effects on biological systems. Examples of how to make QDs biocompatible are silanization, (covering the surface with silane like molecules) and surface exchange with bifunctional molecules. Another strategy is to encapsulate the QD in for instance within phospholipids.
	The functionality of QDs is achieved by giving the surface layer (ZnS) different characteristics. These characteristics enable the QDs to adapt to the desired application by conjugating to a recognition moiety, like antibodies, peptides, oligonucleotides or aptamers. The functionality depends on the biomolecule of interest		The functionality of QDs is achieved by giving the surface layer (ZnS) different characteristics. These characteristics enable the QDs to adapt to the desired application by conjugating to a recognition moiety, like antibodies, peptides, oligonucleotides or aptamers. The functionality depends on the biomolecule of interest

Oyangen: /* Preparation and functionality */

2009-03-26T10:31:17Z

Preparation and functionality

← Eldre sideversjon		Sideversjonen fra 26. mar. 2009 kl. 10:31
Linje 7:		Linje 7:

	===Preparation and functionality===		===Preparation and functionality===
	[[Bilde:qdot.jpg\|left\|thumb\|~~380px~~\|Functionalization of quantum dots]]		[[Bilde:qdot.jpg\|left\|thumb\|400px\|Functionalization of quantum dots]]
	When preparing QDs it is important to keep in mind the physical properties it will get as a finished compound. For instance, biocompatibility, which is the ability of a material to perform with an appropriate host response in a specific application. It is also defined as the quality of not having toxic or injurious effects on biological systems. Examples of how to make QDs biocompatible are silanization, (covering the surface with silane like molecules) and surface exchange with bifunctional molecules. Another strategy is to encapsulate the QD in for instance within phospholipids.		When preparing QDs it is important to keep in mind the physical properties it will get as a finished compound. For instance, biocompatibility, which is the ability of a material to perform with an appropriate host response in a specific application. It is also defined as the quality of not having toxic or injurious effects on biological systems. Examples of how to make QDs biocompatible are silanization, (covering the surface with silane like molecules) and surface exchange with bifunctional molecules. Another strategy is to encapsulate the QD in for instance within phospholipids.
	The functionality of QDs is achieved by giving the surface layer (ZnS) different characteristics. These characteristics enable the QDs to adapt to the desired application by conjugating to a recognition moiety, like antibodies, peptides, oligonucleotides or aptamers. The functionality depends on the biomolecule of interest		The functionality of QDs is achieved by giving the surface layer (ZnS) different characteristics. These characteristics enable the QDs to adapt to the desired application by conjugating to a recognition moiety, like antibodies, peptides, oligonucleotides or aptamers. The functionality depends on the biomolecule of interest

Oyangen: /* Technical limitations in use of QDs↑ From diagnostics to therapy: Prospects of quantum dots; Hassan M.E Azzazy, Mai M.H Mansour, Steven C. Kazmierczak (2007) */

2009-03-26T10:30:53Z

Technical limitations in use of QDs↑ From diagnostics to therapy: Prospects of quantum dots; Hassan M.E Azzazy, Mai M.H Mansour, Steven C. Kazmierczak (2007)

← Eldre sideversjon		Sideversjonen fra 26. mar. 2009 kl. 10:30
Linje 58:		Linje 58:
	The nature of QDs makes us able to monitor different components in a cell culture over extended periods of time. This is used for example in drug discovery, where possible drugs are monitored simultaneously.		The nature of QDs makes us able to monitor different components in a cell culture over extended periods of time. This is used for example in drug discovery, where possible drugs are monitored simultaneously.

	==Technical limitations in use of QDs<ref>↑ From diagnostics to therapy: Prospects of quantum dots; Hassan M.E Azzazy, Mai M.H Mansour, Steven C. Kazmierczak (2007)</ref>==		==Technical limitations in use of QDs<ref>From diagnostics to therapy: Prospects of quantum dots; Hassan M.E Azzazy, Mai M.H Mansour, Steven C. Kazmierczak (2007)</ref>==
	An increased size of QDs due to functionalizations (up to 100nm) limits their ability to reach targets within multi-component molecular complexes. Inherent properties of the QDs themselves may also limit their use as for instance the CdSe/Zns QDs, that do not emit in the infrared region which in turn makes them unsuitable for whole blood analysis. Still they can be used in serum testing and other body fluids.		An increased size of QDs due to functionalizations (up to 100nm) limits their ability to reach targets within multi-component molecular complexes. Inherent properties of the QDs themselves may also limit their use as for instance the CdSe/Zns QDs, that do not emit in the infrared region which in turn makes them unsuitable for whole blood analysis. Still they can be used in serum testing and other body fluids.

Oyangen: /* Technical limitations in use of QDs */

2009-03-26T10:30:35Z

Technical limitations in use of QDs

← Eldre sideversjon		Sideversjonen fra 26. mar. 2009 kl. 10:30
Linje 58:		Linje 58:
	The nature of QDs makes us able to monitor different components in a cell culture over extended periods of time. This is used for example in drug discovery, where possible drugs are monitored simultaneously.		The nature of QDs makes us able to monitor different components in a cell culture over extended periods of time. This is used for example in drug discovery, where possible drugs are monitored simultaneously.

	==Technical limitations in use of QDs==		==Technical limitations in use of QDs<ref>↑ From diagnostics to therapy: Prospects of quantum dots; Hassan M.E Azzazy, Mai M.H Mansour, Steven C. Kazmierczak (2007)</ref>==
	An increased size of QDs due to functionalizations (up to 100nm) limits their ability to reach targets within multi-component molecular complexes. Inherent properties of the QDs themselves may also limit their use as for instance the CdSe/Zns QDs, that do not emit in the infrared region which in turn makes them unsuitable for whole blood analysis. Still they can be used in serum testing and other body fluids.		An increased size of QDs due to functionalizations (up to 100nm) limits their ability to reach targets within multi-component molecular complexes. Inherent properties of the QDs themselves may also limit their use as for instance the CdSe/Zns QDs, that do not emit in the infrared region which in turn makes them unsuitable for whole blood analysis. Still they can be used in serum testing and other body fluids.